R&D Projects | Polymers and Coatings | ELFSE | 2D-CE

End-Labeled Free-Solution Electrophoresis

End-Labeled Free-Solution Electrophoresis (ELFSE) allows size separation of biomolecules using free-solution capillary electrophoresis, similar to capillary gel electrophoresis. In this method a "large" drag-label is attached to the biomolecules to be separated.

We are currently studying the suitability of two drag-label candidates and have one pending patent.

The initial product will be a set of reagents and associated analysis software to be used with existing capillary electrophoresis platforms. However, this method is ideally suited for microfluidic chip systems with shorter separation lengths.

A close-up view of the detector in which a laser is focused to a spot size of 10 microns on the inside of a capillary mounted inside a metal holder block. Fluorescence from separated analytes as they migrate through the capillary during electrophoresis into the laser focus are detected using a 20X microscope lens. Currently this system can "see" a few thousand molecules.

R&D Projects | Polymers and Coatings | ELFSE | 2D-CE